Sentence examples for by multiple PCRs with from inspiring English sources

Detection of HAdV-40 in CSF in this case was confirmed by multiple PCRs with amplicon sequencing.

This study presents a useful method for simultaneous detection of harmful algae by multiple PCR coupled with reverse dot blot hybridization (MPCRDBH).

Additionally, the allelic replacement of the wild-type salKR genes by the SpcR cassette in the suspected mutant was also confirmed by multiple-PCR analysis and sequencing with primers specific to genomic regions lying out the homologous left and right arms (Fig. 3A and 3E).

Our method was carried out with three river samples and three wastewater samples, and the results were compared with those obtained by multiple nested PCR with specific primers for these five species.

The deletion was confirmed by multiple quantitative PCR (qPCR) assays.

The genome was finished by multiple long-range PCRs combined with Sanger sequencing.

This study suggested the feasibility of improving specificity by using of QDs in multiple PCR systems.

Table 1 Primer sets used for the PCR and nested PCR experiments detailed in Fig. 2. As multiple PCR products were obtained by RT-PCR of OPN4 cDNA due to the presence of multiple OPN4 transcripts in 3T3-L1 differentiadipocytescytes, nested PCR was performed to analyze OPN4 transcripts between Exon3 and Exon7.

Contamination or mix-up of extractions, PCR samples or sequence reactions: The sequences are verified by multiple accessions of the species, and multiple PCR and sequence reactions.

PCR/DNA sequencing showed 674 infected by a single high-risk HPV, 188 by a single low-risk HPV, and 136 by multiple HPV genotypes with up to five HPV genotypes in one specimen.

We also tried to investigate the role of other possible factors influencing PCR outcomes by multiple regression analysis.