After standard cell culture of HGFs on these substrates, attached cell number, cell spreading area, immunostained ECM including fibronectin (FN) and type I collage, and vinculin (presenting the formation of focal adhesion plaque) were quantified by morphometric analysis using an immunofluorescence microscope.
Plaque area, was measured by morphometric analysis using an Olympus CCD color video camera attached to an Olympus microscope, and the ImagePro application program calibrated to the microscope objective [39] [41], [46].
Numbers of cells per tubular cross-section were counted for each of the above categories by morphometric analysis using the Simple PCI software (Simple-PCI, Inc). in line with an AX70 light microscope (Olympus, Inc).
The extent of the EB endothelial sprouting was quantified by morphometric analysis using the MetaMorph software.
Lymphatic vessel density was quantified by morphometric analysis using Neurolucida workstation (MicroBrightField, Inc) [ 21], as described (ref [ 11]; supplemental material).
Total cell numbers were counted at 25x magnification and expressed as the number of positive cells per total surface area lung tissue, which was assessed by morphometric analysis using Leica Qwin image analysis software (Leica Microsystems BV, Rijswijk, the Netherlands).
These findings suggest that morphometric analysis using meibography is useful for assessing ocular surface conditions.
GIS-based morphometric analysis using remote sensing and GIS quantitative morphometric have been proved as an efficient tool for hydrological evaluation and drainage network analysis of watershed.
For prioritization of sub-watersheds in water resources management, the morphometric analysis uses some very crucial linear and shape morphometric parameters.
The mesangial volume fraction, defined as mesangial cells, extracellular matrix, and the GBM, was calculated by morphometric analysis adapted from the method in [ 34], using the Fiji Software.
(f) Semi-quantification of NGAL staining by morphometric analysis.
