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Spermatogenesis was evaluated by the enumeration of homogenization-resistant sperm/spermatids in testes and by morphometric analyses of germ cells in the seminiferous epithelium.
Fluorescence [ 39], immuno-fluorescence [ 39] and electron [84] microscopies followed by morphometric analyses of the resulting images have been described elsewhere.
Fluorescence [ 18], immunofluorescence [ 18] and electron [ 80] microscopies followed by morphometric analyses of the resulting images were performed according to established procedures.
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For quantification of mitochondria size by EM, morphometric analyses of mitochondrial length, width, and area were performed from four randomly selected fields per cell with at least 5 cells found in different EM grid sections (80 290 mitochondria) analyzed per group.
For quantification of mitochondria size by 3D-SIM, morphometric analyses of mitochondrial length, width, and area were performed from at least three randomly selected cells (120 270 mitochondria) per group for each experiment, and the experiments were repeated three times.
Differences in growth patterns of the two genotypes were compared by using mathematical models, and morphometric analyses of juvenile salmon were performed to detect differences in body shape.
We first followed the traditional comparative approach by including all 375 specimens in morphometric analyses of total shape (landmarks 1 21), body shape (9 19), head shape (2,7 9,20, 21), and jaw shape (1 6).
Morphometric analyses of A. aegypti FM mitochondria were carried out by TEM using the software AnaliSYS (Soft Imaging System, Germany).
Figure 4 Histologic and morphometric analyses of lesional skin.
Sperm velocity and morphometric analyses of sperm heads and flagella were also assessed.
We present in-depth morphometric analyses of the global and local anatomical features of these arbors.
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