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These findings were confirmed by morphological examination of the cells under a contrast-phase microscope.
The cell preparations contained > 99.9%% granulocytes as determined by morphological examination of > 1,000 cells on Giemsa stained cytocentrifuge (Shandon, Pittsburgh, PA) slides.
However, it is possible to individually determine the sex of the embryos either at early stages by molecular means or late stages by morphological examination of their gonads (Clinton et al., 2001; Chue and Smith, 2011).
Almost 50% of NB patients present at diagnosis with localised disease, that is, they do not have evidence of BM metastases, as assessed by morphological examination of both marrow smears and trephine biopsies, nor other distant localisations investigated by I-MIBG scintigraphy.
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The cells obtained from the solid tumors were single cells or small cell clusters with ≥ 90% viability and with less than 30% contaminating non-malignant cells, as judged by morphological examinations of May-Grünwald-Giemsa-stained cytocentrifugate preparations.
14 The cells obtained were mostly single cells or small cell clusters with ≥90 % viability and with <30 % contaminating nonmalignant cells, as judged by morphological examinations of May-Grünwald-Giemsa-stained cytocentrifugate preparations.
The placentas were conventionally diagnosed as accreta (superficially implanted), increta (within the myometrium), and percreta (through the myometrium) by morphological examination using clear evidence of loss of decidua and the degree of myometrial adhesion as criteria.
Aplidin induced apoptosis in all the cell lines used, as clearly seen by morphological examination by means of sulforhodamine/Dapi staining (data not shown).
Administration of OPP to rats did not affect the well-being of the animals, and no signs of OPP-induced toxicity were observed, as determined by gross morphological examination of major organs.
Species identification of collected material was determined by morphological examination using a taxonomic key to the United States species of Drosophila [ 66].
MSC/HUVEC o-cultures were then monitored every day by morphological examination (under an inverted phase contrast and fluorescence microscope) and quantification of the overall fluorescence in comparison with cultures of only HUVEC (Figure 5B).
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