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The monophyly of the obscura cluster is also suggested by morphological data: in the obscura group, D. dianensis, D. subsilvestris and D. obscura are the only species characterized by pale spots on several abdominal tergites in female [ 30, 40], and large, somewhat quadrate 10th sternite in male [ 30, 41].
The grouping of some Asian mycalesines with the Afro-Malagasy genus Heteropsis is well supported by all combined analyses and independently by the COI and EF-1α datasets (note that the close relationship of M. adolphei with Heteropsis is also supported by morphological data, in particular the form of the male genitalia; [ 7, 18]).
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Previous analyses based on the single SSU gene were likely insufficient and greatly affected by morphological data used in simultaneous analysis [3], [11], [14].
The latter sister relationship is in fact suggested by morphological data as well [ 35], giving more weight to this hypothesis of phylogeny.
In contrast, our molecular data show that Petrocosmea consists of five clades corroborated by morphological data as prementioned.
This relationship is supported by morphological data as well [ 27, 103, 104].
The close relationship to Clitellata as shown by the NucMt data set (Fig. 2) is not substantiated by morphological data.
Light et al. [ 8] and Smith et al. [ 9] used molecular data (18S, EF-1α, and cox1 gene sequences) of species from eight of the 15 families of the Anoplura and is consistent with an early study by Kim [ 10], which used morphological data, in dividing the blood-sucking lice into two major clades.
However, it is now foreseeable that non-invasive imaging techniques, in particular because they offer the opportunity to conduct high-throughput studies and the suitability to analyze whole specimens in a non-destructive manner, together with the broader availability of the respective instruments, will change the way by which morphological data will be acquired and analyzed in the future.
Furthermore, to assess a possible role of epithelial cells in tissue mineralization, we explored the cellular phenotype by correlating morphological data with molecular markers revealed by immunohistochemistry.
The role of morphological variables has also been studied adding to the chemical data set by the morphological data.
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