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The bands were quantified by morphodensitometric analysis using ImageJ software.
The western blotting signal was then quantified by morphodensitometric analysis using ImageJ software (NIH, Bethesda, MD, USA).
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Kinetic parameters (Km, kcat, Vmax) were determined by nonlinear regression analysis using GraphPad Prism 6 software.
ΔΨm was quantified by flow cytometry analysis using a FACScalibur flow cytometer, as described57.
The yield of RNA silencing was assessed by western blot analysis using specific antibody.
This was supported by gene ontology analysis using DAVID27 (Supplementary Table 1).
Statistical analysis was performed by one-way or two-way analysis of variance followed by Bonferroni multiple comparison analysis, using PRISM 7 for Mac (GraphPad software).
Ubiquitination products were detected by performing western blot analysis using substrate-specific antibodies.
All mutations were confirmed by a second, independent sequence analysis using the same primers.
We performed a similar analysis using populations assigned by STRUCTURE from SNP variation.
The researchers did the analysis using DNA microarrays made by the company Affymetrix.
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