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Moreover, by monitoring levels of the hormone melatonin, the team found participants' internal body clocks were shifted to a greater extent when sleep deprivation was combined with lie-ins than if a sleep pattern was consistent.
Liquid chromatography-tandem mass spectrometry (LC MS/MS) has greatly increased the screening possibilities by monitoring levels of amino acids and acylcarnitines.
Child exposure to polycyclic aromatic hydrocarbons (PAH) in polluted air was measured using a biomarker in cord and maternal blood collected at delivery by monitoring levels of air pollution over time.
By monitoring levels of estrogen and progesterone, which drop and rise, respectively, when a woman ovulates, they determined the time of ovulation, down to the day.
The efficiency of subtraction was determined by monitoring levels of the housekeeping gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in these samples.
First, we cultured the VEC+CD31+CD34+CD14− EPs sorted on day 6 with VEGF-A and recombinant human Dll4 (rhDll4) protein (a ligand of the Notch receptor) or the Notch signaling inhibitor DAPT in phase 3. The biological activity of rhDll4 and DAPT was evaluated by monitoring levels of active Notch1 (the Notch1 intracellular domain [N1ICD]) in nuclear extracts of cultured cells by immunoblotting.
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Previously, it was shown that artificially reducing WSTF protein levels by RNA interference, was associated with lower levels of transcription, as measured by monitoring the levels of bromouridine incorporation into RNA [ 18].
We document DsRed regulation both by directly monitoring DsRed mRNA levels using q-PCR and by monitoring florescent levels of the protein using fluorescent microscopy.
These finding were corroborated by monitoring the levels of cyclin B1-mCherry: degradation of cyclin B1 occurred with normal kinetics in the three control conditions but was strongly inhibited in rapamycin-treated cells expressing FKBP-MAD1WT (Fig. 2b).
By monitoring expression levels of these gene sets, the impact of different diets on muscle composition and growth rate can be estimated from a single sample.
The study also evaluated the effects of Fc-OPG on osteoblasts by monitoring serum levels of bone-specific alkaline phosphatase (BSAP), which were largely unaffected by the drug.
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