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Our previous observation that a reduction of DNA binding of NF-Y in cortical lysates of R6/2 HD model mice (15) led us to try to identify novel mutant Nhtt-affected transcription factors by monitoring alterations of their DNA binding in the lysates.
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Imaging studies can also contribute towards determining the efficacy of an agent under development, providing useful information regarding early-treatment response by monitoring alterations in tumour vasculature and angiogenesis (Sessa et al, 2008).
Such degradation was gauged by monitoring alteration in molecular weight, as well as in the content of carbon dissolved and CO2 evolved during composting.
Early recognition of nerve injury, when small fibre loss prevails and staging of advanced disease associated with severe loss of all fibre classes seem promising by monitoring specific alterations of the two quantitative imaging markers observed in this study.
Current expression profiling primarily monitors alterations in steady-state levels of mRNA, but does not address the equally critical issue of whether the proteins encoded by the mRNAs are actually synthesized.
We infected each such well in each set with one of the 10,100 Mtb mutants and monitored alterations in fluorescence induction by automated microscopy.
To monitor alterations in autophagic flux mediated by TMV-RNA, an immunoblot-based LC3 flux assay using flow cytometry was performed to quantify the number of autophagosomes.
The dams were allowed to deliver and their offspring were observed for alterations in development by monitoring a number of gross behavioral, histological and biochemical parameters at newborn, weanling, puberty, adult and geriatric stages.
By monitoring the absorption alterations at 471 nm we assess the activity of chlorophylls, as shown in Table 4.
The effects of such treatments on the induction of cellular apoptosis have been evaluated by monitoring cell cycle alterations and caspase involvement.
We did this by monitoring potential kinetic alterations in effector T cell phenotype, function and fate as adoptively transferred effector T cells differentially trafficked through solid tumors, draining lymph nodes (dLNs) and spleens during the course of tumor regression.
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