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The protein samples were separated by molecular weight using SDS PAGE and four gel slices from each lane were processed for LC-MS/MS analysis and protein annotation (Figure 4).
To analyze low abundant proteins, cell lysates were separated by IEF on different overlapping pH gradients (3 10, 4 7 and 5 8) and then size fractionated by molecular weight using 2DGE on different gradient gels.
cDNA was fractionated by molecular weight using preparative DNA gel electrophoresis after SfiI digestion.
The pellicle protein fractions were further separated by molecular weight using SDS-polyacrylamide gel electrophoresis (PAGE).
The 34 KDa protein was detected by molecular weight using serum of scleroderma (SC) patients as a positive standard.
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Proteins of cell lysates or tissue homogenates were separated by SDS PAGE according to their molecular weight using Tris/glycine as electrophoresis buffer and were transferred onto polyvinylidene fluoride membranes (Carl Roth GmbH, Karlsruhe, Germany) using CAPS buffer.
DI was characterised by molecular weight determination using MALDI-TOF mass spectroscopy.
The four analytical gels were separated by molecular weight during SDS-PAGE, simultaneously, using the Ettan™ Dalt six (Amersham Biosciences).
Peptide substrates and standards were custom-synthesized by ProImmune Ltd. (Oxford, UK), and their concentrations were estimated by weight using their theoretical molecular masses.
We then validated their expression by successfully cloning OR2L13 cDNAs from SN RNA and by detecting two bands of the expected molecular weight by using an anti-OR51E1 antibody on human SN extracts.
Functional poly phenylacetylene)s (PPAs) bearing different azobenzene pendants were synthesized in desirable yields and molecular weight by using organorhodium complexes [Rh(diene Cl]2 as catalysts.
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