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It was investigated through case finding, a case control study, sampling potential sources of infection and by molecular typing (using sequence-based typing (SBT) of all Legionella pneumophila serogroup 1 (Lp1) isolates).
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All isolates were characterized by antimicrobial susceptibility testing, ESBL-production by double-disk synergy test, molecular typing using BOX-PCR, whereas selected isolates were further characterized by beta lactamase and virulence gene content, multilocus sequence typing and phylogenetic analysis.
Nonetheless, molecular typing using only the emm gene versus MLST, yields stratifications that are highly discordant, and neither method by itself is satisfactory for defining strains or clones.
A USA300 strain known as SF8300 [41], which is essentially identical to strain LAC by molecular typing methods, was used to construct single, double, and triple-deletion strains.
Clonal relationships were determined by molecular typing.
The diagnoses were confirmed by molecular typing.
Molecular typing by using randomly amplified polymorphic DNA (RAPD) and antifungal drug susceptibility testing were performed on clinical and environmental isolates recovered from our hospital from 1999 to 2003.
Results of molecular typing by using restriction fragment length polymorphism analysis of rRNA (ribotyping) among 13 clinical and environmental (from seawater and eels in southern Taiwan) isolates of V. vulnificus and arbitrarily primed polymerase chain reaction analysis of 37 isolates (24 clinical isolates and 13 from seawater from coast areas around Taiwan) were previously reported (18, 35).
Isolates from the 2009 2010 and 2012 2013 outbreaks and the isolate from the case-patient from Norway in 2000 were subjected to comparative molecular typing by using a 31-marker multilocus variable number tandem repeat analysis and a broad single-nucleotide-polymorphism analysis.
There are several molecular typing systems used with this aim.
The aims of this study were to identify the bacterial causes of empyema in children by using molecular techniques and to assess the efficacy of PCV7 by using molecular typing of invasive pneumococcal disease serotypes.
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