A significant difference was also obtained for the same 38 isolates when screened by the molecular method using PCR primers (Pearson's χ2 = 12.518, DF = 1, p<0.0001).
Of the 107 Australian Haemophilus isolates that underwent genome sequencing, 18 were not speciated by molecular methods, 15 were speciated using 16S rDNA sequencing [ 16], and the remainder underwent molecular speciation using hpd-based methods [ 22, 27].
Briefly, tracheobronchial aspirates performed at the time of intubation were assessed by culture, by indirect immunofluorescence assay or by molecular methods (PCR or RT-PCR) using previously described procedures [ 14- 17].
Unidentified ticks were conclusively identified by molecular methods developed at UNTHSC, which used amplification of 12S rDNA (Table 1) and sequence determination (data not shown).
The ligand free structure was determined by molecular replacement method using N- and C-domains of the ligand bound RolR as the molecular probe, respectively.
These traditional methods have been mostly replaced by molecular methods in recent years, i.e. using RT-PCR and nucleotide sequencing of the HA and NA genes.
The energy of the isolated iron-nickel nanocluster was calculated by molecular mechanics method using Lennard-Jones potential.
The initial coordinates of atoms in the structures under investigation were determined by molecular mechanics method using HyperChem7 program taking into account electrostatic and disperse forces between the cluster host (silica, polymer) and the guest molecule (articaine).
The structure of the GAD6765loop mutant was solved by molecular replacement method using PHASER [ 22].
Both structures were solved by molecular replacement method using the program MOLREP [15].
