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Free erythrocyte protoporphyrin (FEP) was quantitatively determined by molecular fluorometry using a spectrofluorometer [ 39, 42].
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DNA samples were diluted to 50-100 ng/uL and then quantified in duplicate by fluorometry using PicoGreen™(Molecular Probes, Eugene, OR, US).
The quantity recovered was determined by fluorometry using SYBR Green II (Molecular Probes, Leiden, The Netherlands).
The CuAO activity of the recombinant fusion-proteins (AtCuAO1-MYC9-His6, AtCuAO2-MYC9-His6, AtCuAO3-MYC9-His6) was assayed by measuring the formation of H2O2 by fluorometry using the Amplex-Red hydrogen peroxide assay according to the manufacturer's instructions (Invitrogen Life Technologies).
DNA was quantified for quality control by fluorometry using PicoGreen (Invitrogen).
The N-terminal SMN antibody, followed by a secondary antibody conjugated to Cy3 (GE Healthcare, 1∶4000) was used for quantification of calpain cleavage by fluorometry using unsaturated digital scans performed on a Typhoon Trio+ Variable Mode Imager (GE Healthcare).
Detection of residual DNA was performed with the PicoGreen® fluorescent dye assay (Invitrogen Canada, Burlington, ON) and measured by fluorometry using Lambda DNA for the standard curve (Invitrogen Canada, Burlington, ON).
The analysis was performed by fluorometry using freeze dried, dissected muscle specimens as described [ 36].
The total RNA amount was measured by fluorometry using a Qubit™ - Quant-iT™ RNA Assay Kit (Invitrogen, USA).
DNA concentration was quantified by fluorometry using the CyQUANT Cell Proliferation Assay Kit (Invitrogen) and the λ DNA standard (Invitrogen).
Extracted RNA was resuspended in nuclease-free water and quantified by fluorometry using the RNA Qubit assay (Life Technologies).
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