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This recombinant protein can be made by molecular cloning methods with the DNA construct expressed in an E. coli host system and affinity purified for the following in vitro GC activity testing.
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Plasmid FLAG-sPRDM16 was generated from MSCV-PRDM16 by standard molecular cloning methods.
It has been estimated by traditional molecular cloning methods that about 5% of genes in eukaryotic organisms experienced AS [3].
We developed a laboratory module called pClone that empowers students to use advances in molecular cloning methods to discover new promoters for use by synthetic biologists.
All constructs were made using standard molecular cloning methods.
Using protein purification and molecular cloning methods, researchers have detected multiple xylanases in rumen bacteria and fungi [10], [11], [12].
Molecular cloning methods, protein purification methods, primer sequences.
General molecular cloning methods were performed as described in reference [ 19].
Different concentrations of tripterine treated DU145 cells and control cells, with cell lysate 100 μL pre-cooling (configuration according to molecular cloning method) the ice cracking 30 min, extraction of total cellular protein, quantitative protein by Lowry method.
In this study, in the Min River estuary, southeast China, we analyzed two freshwater and two brackish (oligohaline) marshes in a subtropical estuary, where we assessed methanogenic community structure and abundance by molecular cloning, sequencing, and quantitative PCR methods, as well as by means of soil physicochemical characteristics and concentration of methane dissolved in pore water.
Several copies of a sequence adjacent to 1.688 g/cm3 satellite DNA in the Drosophila melanogaster genome have been isolated by molecular cloning.
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