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This drawback can be solved by modifying the protocol steps for nonrepudiation.
Retinal induction was performed by modifying the protocol previously described [5].
However, we were able to reduce the background significantly by modifying the protocol.
Sequencing was performed on an Illumina GAIIx by modifying the protocol for paired-end sequencing with an index read.
Probe sequences used for each target miRNA are given in Table 4. Northern blotting to confirm novel miRNA tgu-miR-2954-3p was performed by modifying the protocol of [ 97]. 2 μg of total RNA was heated at 65°C for 5 min with 2X loading dye (Ambion), quenched on ice, and loaded on a 15% TBE Urea gel (Invitrogen).
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Glucose production was determined by modified the protocol described by Foretz M et al. [ 39].
Control and communication are decoupled by introducing an upper-bound on the transmission rate, and optimal usage of communication bandwidth and energy is guaranteed by modifying the wireless protocol.
The approaches in [2 5] based on the AP's polling can be implemented by modifying the PCF protocol; however, in the approach in [5] the assumption that the AP knows each ACK frame transmission time beforehand makes piggybacking the uplink data frames on the ACK frames difficult.
Here, we showed that by modifying the standard protocol, the LP were distinguishable.
ChIP assays were performed by modifying the Upstate Biotechnology protocol, as described previously [ 33] except adding 40 mM β-glycerophosphate and 1 mM sodium fluoride to immunoprecipitation buffer, utilizing rat monoclonal antibodies against RNAPII Ser2P (3E7C7, 5 μg; Figure 2B and Table 1) and RNAPII Ser2P (3E7C7, 5 μg; Figure 2B and Table 1).
Nevertheless, future studies should examine what factors contribute to attrition and explore whether attrition can be reduced by modifying the intervention protocols.
More suggestions(16)
by altering the protocol
by modifying the procedures
by modifying the proposed
by modifying the eligibility
by modifying the composition
by modifying the side
by modifying the calibration
by modifying the energy
by modifying the center
by modifying the image
by modifying the expression
by modifying the form
by modifying the imbibition
by modifying the acetylation
by modifying the reflector
by modifying the leader
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