To overcome these drawbacks, Anitua developed plasma rich in growth factors (PRGF) by modifying the procedure of PRP preparation [2].
In order to prepare the key electrophilic component N-benzoyl-α-amido sulfones 4 for the final reaction, a three components reaction involving aromatic aldehyde, benzamide and sodium sulfonate was used by modifying the procedure reported by Chemla [16].
A uniform dispersion of ultrasmall Nb2O5 NPs with a quantum size of 2 to 5 nm is further obtained by modifying the procedure with acetic acid as a stabilizer.
The synthesis of Cu2– x Se nanocrystals was performed by modifying the procedure introduced by the Swihart group.
Native proteins were separated by modifying the procedure of Laemmli (12), where gel electrophoresis was performed under non-reducing and non-denaturing conditions.
By modifying the procedure for in utero electroporation of rodents, we recently created a procedure for in utero electroporation to express transgenes in post-mitotic neurons of the ferret cerebral cortex (Kawasaki et al., 2012).
We have also presented a method to control the orientations of the BTO films either by controlling the thickness of the buffer layers or by modifying the deposition procedure.
In order to investigate irradiation effects by nuclear energy deposition on the number of interstitial sites accessible for He, disordered glasses representative of structures submitted to series of displacement cascades, were prepared by modifying the preparation procedure and the number of interstitial site changes were analyzed.
Small RNA species (including miRNA) were also extracted by modifying the PAXgene procedure by harvesting the flow-thorough of the PAXgene RNA column, and isolated using the miRNeasy Mini Kit (Qiagen), according to the manufacturers' protocols.
By modifying the sampling procedure it is possible to simulate the evolution (i.e., increased substrate uptake and growth rate, increased fitness) of an organism in a particular growth environment.
