Sentence examples for by modifying the previously from inspiring English sources
The pathway was designed by modifying the previously reported (R -1,3-butanediol synthetic pathway to consist of pct (pR -1,3-butanediolnsfeR -1,3-butanediolhaera elsyntheticktB (thiolase), pathwayADPH-dependentoaconsisttyl-Cof reductase) from Ralstonia eutropct, bld (butyraldehyde dehydrogenase) from Clostridium saccharopropionateetonicum, and the endogenous alcohol dehydrogenase(s) ofrom coli.
Constructs were prepared by modifying the previously published VSFP2A and VSFP2.1 plasmids6.
The SB T2/OncZ transposon was built by modifying the previously characterized T2/Onc vector [10] by inserting the carp ß-actin promoter [33] (detailed cloning steps are provided in Methods S1.
TEM observation was performed by slightly modifying the previously described sample preparation method (Asahi et al. 2014).
Retinal induction was performed by modifying the protocol previously described [5].
For evaluation of osteochondral defect repair, we designed a new histological grading score (Table 1) by modifying the scoring system previously reported by us (Wakitani et al. 1994).
An interactive FL-mapplet was then generated by modifying the data in the previously reported MQN-mapplet application [40].
By modifying the conventional SOM, we have previously developed Batch-Learning SOM (BLSOM), which allows classification of sequence fragments according to species, solely depending on the oligonucleotide composition.
ChIP assays were performed by modifying the Upstate Biotechnology protocol, as described previously [ 33] except adding 40 mM β-glycerophosphate and 1 mM sodium fluoride to immunoprecipitation buffer, utilizing rat monoclonal antibodies against RNAPII Ser2P (3E7C7, 5 μg; Figure 2B and Table 1) and RNAPII Ser2P (3E7C7, 5 μg; Figure 2B and Table 1).
Here, we constructed a novel butyrate synthetic pathway that functions under aerobic growth conditions in E. coli, by modifying the 1-butanol synthetic pathway reported previously.
Reduction of the radiated noise of an axial piston pump by modifying the piston pump shell structure has previously been achieved.
