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This approach consists in introducing a wavefront curvature Φlens x,y) on the beam incident on the SLM by modifying the alignment of the first beam expander.
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Therefore, mapper robustness could probably be improved by modifying the scoring parameters in the alignment step by decreasing the indel penalty.
This was achieved by modifying the pattern inclination using a simple user interface and validating the alignment by live monitoring of the recorded count rate.
The increase in the alignment rates shows that extra information was incorporated in the pseudo-molecule sequences by modifying the SNP and InDel sites.
Twelve models simulating four different sizes of necrotic lesion (0°, 60°, 100°, 115°) and three different alignments of the femoral component (10° varus, neutral, 10° valgus) were created by modifying the intact model.
We modified the alignment graph to store the relative orientation of the matches as reported by Nucmer for each edge.
The user can manually modify the alignment, if needed.
A user/annotator is in control of the alignment process and confirms, rejects or modifies the alignments proposed by ILink.
By modifying our alignment protocol to systematically detect pseudo-templated nucleotides at transcript 5′ ends, we observed that up to 15% of S. agalactiae TSSs were associated with a reiterative transcription (Additional file 2).
It is our goal to modify the pairwise alignment problem in a way that we can prove the resulting alignment score to be pd.
We manually modified the multiple alignment output from ClustalX with the Bioedit program [ 45].
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