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The R. ferrireducens metabolic network was reconstructed in SimPheny (Genomatica, Inc., CA) by modifying previously published procedures [ 56].
The procedure for isolating rat adrenal cells was developed by modifying previously published methods (Fredlund et al., 1975).
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Procedures were developed by modifying previously described protocols [23], [11].
To address this, we first engineered monodisperse FN nanofibers with a ribbon-like morphology by modifying a previously published SIA technique18,19.
Constructs were prepared by modifying the previously published VSFP2A and VSFP2.1 plasmids6.
Medium spiny projection neurons (MSNs) were also generated from E14.5 ventral forebrain derived NSCs by modifying a previously published method [104].
Protein extraction from purified brain vessels was performed by modifying a previously published protocol [ 30].
Myobundles were formed by modifying our previously published methods for engineered rodent muscle tissues (Hinds et al., 2011; Juhas et al., 2014).
Furthermore, we described fabrication of myobundles in more detail, as follows, in the Materials and methods section: "Myobundles were formed by modifying our previously published methods for engineered rodent muscle tissues (Hinds et al., 2011; Juhas et al., 2014).
A stoichiometric network model of adipocyte central carbon metabolism was formulated by slightly modifying a previously published model [ 15].
To assess the impact of the J-domain and UIM domains on the suppression of mutant huntingtin aggregation by human HSJ1a, we modified a previously published in vitro assay (Scherzinger et al., 1999; Tam et al., 2009) to quantify GST-HTT Q51 aggregation in the presence of purified HSJ1a or different SK-N-SH cell lysates and an ATP regenerating system.
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