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The saponification of waxes was carried by modified procedure of Morrison and Smith [17], dissolving the waxes (0.25 g) in benzene/methanol (1 1, 10 ml), followed by the addition of 95% ethanol (25 ml) and 60% aqueous potassium hydroxide (5 ml) and refluxed for 8 h [15, 17].
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Non-saponifiable lipids for sterol analysis by HPLC were isolated by the modified procedure of Breivik and Owades [26].
In vitro, one step abomasum and ileum digestibility were followed by the modified procedure of Boisen and Fernhndez (1995), by utilizing undigested Rusitec residue for abomasum digestibility, and for further ileum digestibility, the abomasum residue was utilized.
Solid-state incorporation was performed by a modified procedure of Refs.
Therefore, the dinuclear zinc II) complex of tetraiminediphenol macrocycle has been synthesized by the modified procedure of Garcia et al. [35] where the [2+2] template condensation is carried out between 2,6-diformyl-4-methylphenol and 1,2-diaminobenzene in the presence of zinc II) nitrate hexahydrate in methanol.
The dipeptide L-Ala-D-Glu was synthesized chemically according to Sachs and Brand [46] and the tripeptide L-Ala-γ-D-Glu-L-Lys was synthesized by a modified procedure of Schmidt et al. [47].
TNBS-ileitis was induced by laparotomy according to a modified procedure of Pontell et al. [ 29, 30].
Anthocyanins were isolated following a modified procedure of that described by Renault et al. [ 19].
This was done using a modified procedure of the method described by Longin [20].
Spleen samples obtained in 2006 and 2007 were used for detection of BTV RNA segment 5 and cellular β-actin transcripts by reverse transcription quantitative PCR (RT-qPCR) according to a modified procedure of Toussaint et al. (2 ).
The synthesis of CdSe and CdSe/ZnS NPs was undertaken following a modified procedure of Peng et al. [25].
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