Sentence examples for by modification of plasmid from inspiring English sources

SM636 was engineered by modification of plasmid SM353 with primers 5055/5056 and 5057/5058 by site-directed mutagenesis as in Scott et al. (2006).

The transformation vector diagrammed in Figure 1A was obtained by modification of the plasmid ptrnI-RT [ 60].

Independent studies in bifidobacteria have demonstrated that following modification of plasmid DNA, R-M systems can be by-passed, thereby resulting in a substantial increase in transformation efficiency and in some cases the successful generation of mutants [ 15, 20].

Plasmid DNA is highly stable and flexible, allowing for the modification of plasmid sequences [ 14].

Plasmid pT7-DsRed-NLS is a modification of plasmid pSP6-DsRed-NLS (Price et al., 2010), in which the SP6 promoter is replaced with the T7 promoter for higher-yield transcription, and the coding sequence of interest is flanked by the β-globin 5′ and 3′ UTRs.

For selection of transfectants, an Escherichia coli TS cDNA under the control of a murine stem cell virus LTR (long terminal repeat) promoter, optimized for expression in mammalian cells by modification of codon-usage, was included in the plasmid [ 28].

CAN1 shuttle vector plasmids were created by modification of pBL245-based shuttle vectors described previously.

In addition, the expression level of the plasmid could be controlled by modification of the growth conditions or by utilisation of promoters of varying strength.

In the present paper, we improved the yield of this product by modification of the − 35 region of the rrn promoter so as to escape from the Fis protein control and the use of a new vector plasmid.

In addition, it was found that modification of the plasmid did not significantly affect the pCA2.4 per chromosome copy number.

Site-directed mutagenesis of genes and the modification of the plasmids were performed by inverse-PCR followed by phosporylation and self-ligation using T4 polynucleotide kinase and T4 DNA ligase.