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The tolerant pool (T-pool) was made by mixing equal amounts of DNA from 20 extreme cold-tolerant RILs with a PSST above 0.90, and the sensitive pool (S-pool) was made by mixing equal amounts of DNA from 20 extreme cold-sensitive RILs with a PSST below 0.63 (Additional file 2: Table S1).
We used several standard controls on all plates that were genotyped, including the reference genome line Nipponbare, 93-11, an F1 between Nipponbare and 93-11 (kindly supplied by Guo-liang Wang of Ohio State University) and a "pseudo F1" that was prepared by mixing equal amounts of genomic DNA from Nipponbare and 93-11.
Samples for the initial NMR experiments were prepared by mixing equal amounts of the mineral leachate and a trifluoroacetic acid/fluoride-containing solution.
Monoclonal mouse anti-human IgG was prepared by mixing equal amounts of mouse anti-human IgG1 (51 mg/ml), IgG2 (22 mg/ml), IgG3 (16 mg/ml) and IgG4 (24 mg/ml).
In brief, the in-house reference pool was created by mixing equal amounts of total RNA extracted from adipose tissue samples of subjects undergoing plastic surgery, as previously described [21].
Total RNA was extracted separately for each tissue type used, including heart, gill, nerve, muscle, and whole crabs, and a pooled total RNA was constructed by mixing equal amounts of total RNA from each of the tissue specific pools.
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Chimeric pairs were prepared by mixing equal amount of NC105.1-RFP NC105.1-RFPer wild strand in the experiment set, i.e. NC28.1, NC63.2, other85.2, wilde control pairstrain prepared by mining equal amounthef NC105.1-RFP and NC105.1.
The leaf samples of each accession were collected by mixing equal amount of leaf tissues from 6 plants from National Germplasm Guangzhou Sweetpotato Nursery located in Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
For cDNA library construction, fruit and flower RNAs were pooled, respectively, by mixing equal amount of RNA from each developmental stage.
By mixing equal amount of RNA of the four stages, RNA pools from both QS and EG were established in parallel.
For an initial scan of the whole marker set, a DNA pool was established for each group by mixing equal amount of genomic DNA prepared from individuals within each group.
More suggestions(15)
by adding equal amounts
by using equal amounts
by mixing different amounts
by mixing equal masses
by mixing adequate amounts
by plating equal amounts
by placing equal amounts
by mixing equal quantities
by mixing substantial amounts
by mixing variable amounts
by mixing certain amounts
by mixing various amounts
by mixing small amounts
by mixing stoichiometric amounts
by injecting equal amounts
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