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Aqueous two-phase systems were prepared at room temperature by mixing certain amounts of 50% (w/w) of PEG solution, 40% (w/w) of phosphate buffer, 2 ml cell culture supernatant sonicated in 50 mM of citrated buffer (pH 6.0), and NaCl in 15 ml centrifuge tubes with conical cap.
Aqueous two-phase systems (ATPS) are formed by mixing certain amounts of polymers, salts, and water together, which have been extensively used to separate and purify nuclear acids, cells and organelles, enzymes, and proteins (Asenjo and Andrews 2011; Raja et al. 2012).
For transfection to the human skins in vivo, a pre-made SpRNAi-RGFP vector solution was formed by mixing certain amounts (ie, 1 1000 μg) of the purified SpRNAi-RGFP vector with or without either anti- EGFP, miR-Tyr or miR-Hyal pre-miRNA insert in 1 ml of autoclaved ddH2O with 99 ml of 100% DNase-free glycerin (or glycerol).
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The modified carbon pastes were obtained by mixing a certain amount of Pd/SWCNTs@MOF-199 or MOF-199 (as modifiers) with binder and graphite.
The first agents used as heavy tamponades (FSi, PFCL, and F6H8) have low viscosity and fast intraocular emulsification; however, the resistance to extensional deformation and therefore the extensional viscosity of F6H8 may be increased by mixing a certain amount of very long-chain silicone molecules into the heavy tamponade.
Mayenite support was produced by mixing stoichiometric amounts of calcium hydroxide and aluminium nitrate.
Composites were prepared by mixing appropriate amounts of the two solutions inside 1.5 mL vials.
The microemulsions were prepared by mixing appropriate amounts of isooctane, AOT, and ethylene glycol.
Cordierite was mechanochemically synthesized by mixing proper amounts of kaolin, talc and Al(OH 3.
The Spx-αCTD complex was formed by mixing equimolar amounts of purified C10S Spx and αCTD.
Pools were obtained by mixing equal amounts of total RNA from each individual sample.
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