Ordering genes by the minimum of expression found many motifs, 38, overrepresented at points that together span the whole life cycle (no motifs were found across 100 permutations with the minimum expression data randomly ordered).
For example, the large number of motifs overrepresented throughout the life cycle when genes were ordered by the minimum of expression indicates that these motifs correspond to points in the life cycle when expression is at the lowest and thus may be associated with repressor mechanisms.
MRP3 could be an important target for immunotherapy, if the expression of MRP3 RNA by gliomas and minimum of expression in normal brain is reflective of actual MRP3 protein expression.
Quite a number of conserved motifs were found in co-regulated genes, whether when ordering genes by the maximum or minimum of expression.
Reg3α was down-regulated with a minimum of expression at 7 h, followed by an increase which was maximal at 7d (Fig. 3A).
It is also bounded above by $1$, and thus by the minimum of those expressions.
These filters operate by removing genes from the co-expression analysis that fail to show a minimum ratio of expression change (R) in a minimum number of microarray experiments (M), thereby eliminating the genes most likely to be unresponsive in the array set being analyzed.
After expression summary calculation all gene expression profiles were minimized by subtraction of the universal minimum of all expression values.
The data were further analysed by imposing one or all of the following selection criteria: (1) a minimum level of expression was observed; (2) significant differential expression (twofold or greater) was observed; and (3) the expression pattern was reproducible.
To further characterize the differences in the gene expression patterns, we examined the range (maximum and minimum) of the expression values exhibited by all alternative growth conditions.
