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Thus a negative malaria result diagnosed by microscopy would be more reliable than a positive result.
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The PSA found that the effect in terms of increased number of correctly treated fever patients was not significantly different from zero if microscopy would be replaced by RDT diagnosis.
Thus, a direct comparison of these lines by DIC microscopy would effectively be an uncontrolled experiment since there is no satisfactory way to control for exposure time between the two transgenic lines with differing basal expression.
Labelling cells fluorescently, either through the use of a dye or by transfection with a fluorescent reporter construct, would facilitate tracking of cell movement by microscopy, but such a technique would be difficult in organotypic cultures grown on grids.
By isolating and determining the composition of PB in differential assembly conditions, we have identified components that would be undetectable by microscopy either because of low abundance, transient interactions, or association in nonstress conditions when foci are undetectable.
Such a dot transparency would be easily detected by microscopy systems dedicated to biological samples that are known to produce relatively weak contrasts.
The results shown here would be further corroborated by microscopy showing the distribution of the two gellan types within the mixed system.
Since we were aiming to detect L-SR junctions, namely close appositions of the lysosomal and SR membranes, immuno-gold labeling of lysosomes was prohibited, since this technique compromises membranes definition by electron microscopy to the extent that we would be unable to assess junctional architecture.
Nevertheless, if the scaling of domain size with lattice size were exact, ∼3/4 of the lipid in a GUV with a composition BSM/Chol/POPC ∼1 1 1 would form a domain that would be visible by fluorescence microscopy.
This would be readily evaluated by confocal microscopy in permeabilized cells.
These sizes would be below the resolution of fluorescence microscopy.
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