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Field samples negative for any of the target species by microscopy, were also negative by PCR.
All sediment samples that contained any of the target species as confirmed by microscopy, were also positive for PCR.
Patients with no confirmation of diagnosis by microscopy were also excluded.
All samples positive for D. fragilis by microscopy were also positive by PCR.
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Among the 624 persons sampled during the cross-sectional survey the sex ratio was 50/50, but because infections were more common in men, the random subsample of 37 for PCR analysis of the 49 positive results by microscopy was also male biased.
Accordingly, images of all CTCs identified on patient cytospins and representative images of 100 cells from each MCF-7, T47D, MDA.231 and Hs578T cell lines obtained by confocal microscopy were also assessed by ImageJ.
Samples for cell counts (1 mL for HC treatments, 10 mL for LC treatments) by fluorescent microscopy were also removed at the beginning and end of the experiment, and immediately preserved with 2% formaldehyde and refrigerated until processing.
Paraffin embedded tissues from individuals who had normal liver histology (n = 4) as determined by light microscopy, were also included as controls in the immunohistochemistry experiments.
Other methods, such as visualization of LC3 puncta by microscopy and the "gold standard" of autophagosome visualization by electron microscopy are also widely used to measure autophagy.
Ex vivo histochemical analysis of ROS by fluorescence microscopy was also performed in J. multidentata gills, indicating a significant increase upon exposure to 10 μg L−1 AZM.
A set of photomicrographs by light microscopy is also presented in Figure 2, which shows the representative medial tibial plateaus in the three groups.
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