Sporozoites were detected by microscopic screening in 89 (1.57%) of 5,663 dissected An. dirus mosquitoes, and oocysts were observed on 0.94% of the midguts.
The collected samples were processed by the formalin-ether concentration technique [ 47], followed by microscopic screening aided by Lugol's iodine stain.
Culture conditions selected based on optical microscopic screening of B16F10, Mϕ/Ly and B16F10/Ly-Mϕ B16F10/Ly-Mϕuntreated), were orserved by SEM.
Using light microscopic screening, no signs of other extraglandular disease manifestations or other independent autoimmune diseases were found.
In all experiments female individuals, screened by microscopic inspection of gonads, were used for subsequent analyses.
Newborns of infected mothers are screened by microscopic examination of cord blood after concentration (microhematocrit, Strout's method) and, if negative, by PCR.
Participants were screened for malaria both by microscopic examination of Giemsa stained blood smears as well as by a rapid serological assay (Paracheck Pf (P. falciparum) Rapid Test (Dipstick) supplied by Orchid Biomedical Systems.
Furthermore, all human landing catchers were screened weekly for malaria by microscopic examination of thick smear peripheral blood samples and, when found infected, treated with artemisin-based combination therapy.
Stool samples were obtained at enrolment and annually from each child and screened for eggs and parasites by trained parasitologists both by microscopic observation of stools, as well as after using the eggs concentration technique described by Bailenger [24].
Urine outcomes: creatinine (Hitachi 747, Roche Diagnostics GmbH, Mannheim, Germany); micro-albumin (Beckman Array 360 system); and β2-microglobuline (IMx Abbott); screening for protein, glucose, pH, blood and leukocytes (teststrip Boehringer Mannheim B.V ., followed by microscopic evaluation of the urinary sediment if indicated.
