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Expression of the Beclin-1 constructs was confirmed by western blotting, and induction of autophagy was measured by microscopic monitoring of GFP-LC3 dots.
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The maximal nontoxic concentration (MNCC), defined as the maximal concentration of the extract that did not exert toxic effect detected by microscopic monitoring, was recorded at the end of treatment.
The maximal non-cytotoxic concentration (MNCC) was estimated as the maximal concentration of the samples that did not exert toxic effects by microscopic monitoring.
Each virus also encodes the green fluorescent protein (GFP) for the microscopic monitoring of transduction efficiency and ∼95% of the Schwann cells were transduced, as visualized by the presence of the green fluorescence (data not shown and Figure 1).
Online microscopic monitoring ensured that only one cell was aspirated during the process of single cell picking.
Microscopic monitoring(6), toxicity tests(7), visual inspection.
Cell viability following oxidative insult was monitored by microscopic assessment of cell morphology and cell numbers using a Cell-IQ 2 automated live cell imaging platform (Chip-Man Technologies Ltd).
The approach requires apparent verification of the cellular functions occurring on the ceramic surface, and these functions could be monitored by microscopic observation of the cultured living cells on the ceramic material.
The course of the infection was monitored by microscopic examination of thin blood smears.
The course of parasitemia was monitored by microscopic examination of Giemsa-stained thin smears of tail blood.
Emergence of erythrocytic stages, resulting from complete liver stage development, was monitored by microscopic examination of daily blood smears.
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