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Weights from samples obtained by microdissection are shown in Table 1.
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After manual microdissection, > 60% of the sample area was shown to contain tumor cells as estimated from the H&E-stained H&E-stained
Purification of tumor epithelium by laser microdissection was supposed to give the most reliable tumor-specific gene expression profiles.
The PCR analysis clearly showed that the transplanted epithelial cells excised by laser capture microdissection were C57BL/6 in origin.
RNA from basal and luminal cells isolated from benign tissue by immunoguided laser-capture microdissection was subjected to expression profiling.
Paired normal and tumor specimens from 52 primary prostate cancers, obtained by laser capture microdissection or manual microdissection, were analyzed for PSGR expression by semiquantitative and real-time PCR assays.
The efficiency of the laser capture microdissection was assessed by examining the tissue harvested under the microscope.
Quality of the microdissection was determined by evaluating the expression of glomerular specific genes (NPHS1, NPHS2, and SYNPO).
Accuracy of microdissection was confirmed by microscopic review of post-microdissection stained slides.
The histopathological diagnosis in the frozen sections for microdissection was agreed by two consultant histopathologists.
Tissue microdissection was guided by a lung pathologist to ensure >80% tumor cell or >80% non-malignant cell content.
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