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Synonymous and non synonymous substitutions in mtDNA in all coding and control regions were identified in dorsolateral prefrontal cortex (DLPFC) by microarray resequencing of schizophrenia (SZ), bipolar disorder (BD), major depressive disorder (MDD), and control (C) subjects.
The SNPs were all identically ascertained by microarray resequencing of the genome, and verified in multiple populations.
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In addition it was not observed in 12 mtDNA samples from control blood measured by microarray resequencing, reducing the likelihood of a microarray artifact.
Microarray resequencing of human mtDNA sequences that differ by << 1% from the tiled human reference approach 100% efficiency and accuracy.
Microarray resequencing of chimpanzee and gorilla DNA sequences, which differ by 8 and 10% from the tiled human sequence, recovers ~85% of those sequences, with < 2% error.
The microarray resequencing of mtDNA was 100% concordant with conventional sequencing results for 103 mtDNA variants.
These results indicate that microarray resequencing of the mtgenome recovered from NAF is a rapid, sensitive and cost effective technique.
In this report, we characterized a persistent infection by multiple CTV genotypes by genome-wide microarray resequencing analysis and deep sequencing analysis of selected genomic regions.
Preparation for microarray resequencing includes pooling of amplicons at equimolar concentration, nuclease fragmentation, labeling, and fluorescent staining, according to the Affymetrix GeneChip CustomSeq resequencing Array protocol, v. 2 (2003).
The accuracy of the microarray resequencing process was defined as the ratio of the number of correctly determined bases to the total number of determined bases, by comparison with the known rpoB sequence of the tested strains.
They recommend the cautious use of microarray resequencing as an initial evaluation tool and propose to limit the use of Sanger sequencing to more problematic and discordant cases.
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