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To find the miRNAs that may be involved in dark-induced leaf senescence, we identified miRNAs by microarray platform using Arabidopsis thaliana leaves from both whole darkened plants (DPs) and individually darkened leaves (IDLs).
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Affymetrix GeneChips are a popular oligonucleotide microarray platform, using probe sets formed by 11-20 pairs of 25 mer probes.
The next level of clustering was defined by the microarray platform used for the studies.
The microarray platform used by both databases involved in the validation study contained 22,283 probes set.
The Wang et al. dataset [ 26] includes data from 209 patients, and used the same Hu133A-Affymetrix microarray platform used by Frasor et al. [ 10].
The Alzheimer's Disease module also appeared as differentially activated, most likely due to its hybrid composition (20/22 genes that compose the AD module are present in the microarray platform used by Rubins et al [ 1], and 14/20 are also members of other modules).
Then, we constructed an expression matrix where the number of rows matches the number of genes represented in the microarray platform used by Lecker et al. [ 4] and where there are 5 columns as the number of experiments: 1 experiment for diabete, 2 experiments for tumor and 2 experiments for uremia.
Affymetrix was the most common microarray platform used.
The first step in any meta analysis of microarray data is to find the set of genes which is shared by all microarray platforms used in the analysis.
The set of pathways obtained from the MSigDB covers more than 5,000 distinct genes, where 3,271 of them can be found in both microarray platforms used by the two breast cancer gene expression studies in [10], [11].
Differential effects on macrophage gene regulation between studies might be caused by the different in vitro models, multiplicities of infection, time p.i., and/or microarray platforms used.
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