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To determine correlations between disparate methods of assessing gene expression levels, we selected a cohort of CD antigens as a reference gene set and compared transcript abundance measurements acquired by microarray hybridization with protein abundance measurements determined by immunohistochemistry in specific cell types found in the human prostate gland.
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Waterhouse et al. reported that 80% of the S. mutans ORFs are conserved among ten strains with different serotypes by microarray hybridization based on the UA159 genome, suggesting that 80% of ORFs are conserved within S. mutans strains [ 28].
Genotyping by microarray hybridization has proven to be challenging in species with complex genomes.
DNA was extracted with phenol and chlorophorm and analyzed by microarray hybridization (as above) or qPCR as described below.
Similar results were obtained with the 636 immune-related transcripts estimated by microarray hybridization.
These data are consistent with the differential expression patterns of these genes detected by microarray hybridization analysis.
Albert, T.J. et al. Direct selection of human genomic loci by microarray hybridization.
The samples were analyzed by microarray hybridization and quantitative PCR.
A typical example of the raw signal intensity data sorted by organism annotation is shown for microarray hybridization with B. subtilis targets (Fig. S1).
Overall, the expression patterns of all the 13 genes analyzed by sqRT-PCR were consistent with those by microarray hybridizations [see Additional file 11].
Details regarding differences in ORF composition, detected by the microarray hybridizations with all Xf strains analyzed in this study, can be found in Additional File 1.
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