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The cDNA synthesis and in vitro transcription amplification were followed by microarray hybridization using the Human HT-12 v.4.0 Expression BeadChip kit following the manufacturer recommended protocols (Illumina).
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Specificity of BAY 87-2243 for the suppression of HIF-1-mediated gene transcription on a genome-wide scale was evaluated by microarray hybridizations using Affymetrix whole genome 133Av2 arrays.
We also carried out a genome-wide gene expression analysis in normal and cancer cells of breast and prostate by DNA microarray hybridization, using Illumina's Sentrix Human-6 Expression BeadChips.
As a consequence, in microarray hybridizations using this amplified RNA, these motifs are abundantly present.
Gene transcription changes between the cold-acclimated and normal samples were obtained by microarray-based hybridization using three sets of RNA samples prepared from fishes of three acclimation experiments.
To assess transcript classification by microarray hybridization, qRT-PCR was used.
Quantitative PCR was performed on 11 genes found to be differentially expressed by microarray hybridization as well as a housekeeping gene used as an internal control (bovine ribosomal protein L19; RPL19).
Analyzing MuHV-4 lytic transcripts by microarray hybridization [9] [11] has been of limited use for ORFs 75a/b/c, because latency associated ORF73 mRNAs splice across them [12].
Diluted samples were then used in multiplex RT-PCR, followed by microarray hybridization.
One commonly used approach to detect regulatory interactions between TFs and genes is chromatin immunoprecipitation followed by microarray hybridization (ChIP-chip)[1], [2], which is a binding assay.
The samples were analyzed by microarray hybridization and quantitative PCR.
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