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The expression profile of the maize IBM2 Syn10 double haploid population [ 19] was determined by microarray hybridization to 60-mer probes.
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To test if the isolated cDNAs are expressed in the manner expected (i.e. are induced by one or more of these polysaccharides but absent when grown on glucose) we selected unique cDNAs representing 728 contigs and hybridized them by conducting single-channel, slide-based microarray hybridization to labeled cDNAs made from fungal cultures grown on individual polysaccharides.
With regards to matrix proteins, differences in levels of CILP mRNA detected by microarray hybridization correspond to previous reports of protein accumulation in the extraterritorial matrix of adult cartilage with age [ 49].
Genotyping by microarray hybridization has proven to be challenging in species with complex genomes.
Pacing-induced alterations in the expression of other miRNAs with particular concern for their involvement in AF could not be determined by microarray hybridizations due to a high variability among replicates (miR-1, miR-21, miR-23a/b, miR-29a, and miR-133a) or very low hybridization signals (miR-10a, miR-10b; see the complete microarray data at NCBI through GEO accession number GSE65330).
DNA microarray analysis revealed that GR5 is highly divergent; its genes were not sufficiently similar to be detected by microarray hybridization in any other Cronobacter strains.
Quantitative PCR was performed on 11 genes found to be differentially expressed by microarray hybridization as well as a housekeeping gene used as an internal control (bovine ribosomal protein L19; RPL19).
To assess transcript classification by microarray hybridization, qRT-PCR was used.
To confirm the miRNA composition determined by microarray hybridization, we measured the cellular and extracellular mature miRNA populations by quantitative PCR approaches after reverse-transcription of miRNAs (qRT-PCR).
Correlations of log2 fold-change values in response to UV and AgNO3 treatment obtained by microarray hybridization are shown in Figure 2.
Mean values of fold difference for each cohort were compared to mean values obtained for pools analyzed by microarray hybridization.
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