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The next two columns display fold enrichment of ChIP sample over input detected by microarray hybridization or real-time PCR, respectively.

These methods monitor either Spo11 binding (via chromosome immunoprecipitation followed by microarray hybridization or sequencing, ChIP-chip, or ChIP-Seq) or the ssDNA tail left by Spo11 at the DSBs (via benzoyl-napthoyl-DEAE-cellulose ssDNA enrichment).

A decade of work with yeast and other microbes has shown that DNA barcodes are sensitive and quantitative genetic markers that permit cell-based screening in high complexity pooled formats, with subsequent deconvolution by amplification of barcodes followed by microarray hybridization or high throughput DNA sequencing [ 19- 21].

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Plasmid-encoded genes involved in conjugation and mobilization as well as replication and maintenance were assessed by microarray hybridization analyses or, for a few targets, by PCR (Additional file 3: Figures S2A and Additional 5: Figure S3A).

DNA was extracted with phenol and chlorophorm and analyzed by microarray hybridization (as above) or qPCR as described below.

Examples of the former include immunoprecipitation followed by microarray hybridization (RIP-Chip) or sequencing (RIP-Seq) [ 8, 14, 15].

Modern high-throughput techniques, such as chromatin immunoprecipitation (ChIP) followed by microarray hybridization (ChIP-chip) or by massively parallel sequencing (ChIP-Seq), allow genome-scale mapping of TF occupancy in a given cell type and state [ 1].

Chromatin immunoprecipitation (ChIP) followed by microarray hybridization (ChIP-chip) or high-throughput sequencing (ChIP-seq) allows genome-wide discovery of protein-DNA interactions such as transcription factor bindings and histone modifications.

After the amplification of the targets the detection can be performed either by capillary electrophoresis or by microarray hybridization.

Finally, some studies have characterized a few Photorhabdus and Xenorhabdus RGPs by in silico analysis [ 35, 36, 39- 43] or by microarray hybridization [ 38, 44].

The presence of cagA as a biomarker for cag PAI and gastric colonization with cagA+ strains associated with gastrointestinal diseases has been documented by PCR in isolated bacterial DNA and has also been detected by hybridization in situ from biopsies or by microarray hybridization [ 4, 5, 10- 12, 14- 16, 24, 25].

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