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Correlations of log2 fold-change values in response to UV and AgNO3 treatment obtained by microarray hybridization are shown in Figure 2.
The Sanger Institute's annotation and publication of the complete genome of a different strain of N. lactamica may reveal that some particularly divergent gene families that appear to be absent by microarray hybridization are actually present in N. lactamica, but are too divergent to be detected by our microarray probes (as in Table 3).
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Changes detected by microarray hybridization were confirmed by real time PCR for Acly, Kat2a, Dhx28, and Stat5a (data not shown).
The expression patterns of these genes measured by qPCR or microarray hybridization were generally similar.
Comparing expression profiles contained in both analyses performed on identical total RNA, we found that the expression profiles assessed by qRT-PCR or microarray hybridization were highly similar.
DNA labelling and microarray hybridization was conducted by staff at the W.M. Keck Facility at Yale.
The signal values of microarray hybridization were normalized by the probe logarithmic intensity error (PLIER) algorithm.
RNA labeling and microarray hybridization was conducted by CapitalBio Corp (Beijing, China).
Microarray hybridization was performed by the University of Southern California Affymetrix MicroArray Core Facility at Children's Hospital Los Angeles.
Microarray hybridization was performed by NimbleGen Service Facility (Iceland) according to the manufacturer's procedures.
RNA isolation, purification and microarray hybridization were conducted by the Phalanx Biotech Group.
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