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In brief, concentration levels of approximately 300 oral taxa were determined by microarray hybridisation using a fluorescent readout reverse-capture method [ 34].
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Huang [ 11] successfully validated differential expression of 15 genes identified by cross-species (mouse on human microarray) hybridisation using reduced stringency.
Isolates which appear to be indistinguishable by, e.g., PFGE might yield differences detectable by microarray hybridisation or genome sequencing.
Consequently, a MLST clonal complex can be identified by microarray hybridisation based on a characteristic fingerprint pattern [96], provided that a hybridisation pattern for this CC has been defined previously.
Genes encoding leukocidins and exfoliative toxins as well as protease genes splA, splB and splE cannot be detected by microarray hybridisation.
For microarray hybridisation we used a common reference design as proposed by Dudley et al. [ 68], in which a fluorescence labelled antisense oligonucleotide (complementary to a sequence tag present in all spotted microarray probes) is hybridised together with the labelled cDNA of interest.
FGFR1 amplification was analysed in tissue microarrays comprising a cohort of 880 unselected breast tumours by means of chromogenic in situ hybridisation using inhouse-generated FGFR1-specific probes.
To obtain RNA for microarray hybridisation, we used the following procedure.
To generate fluorescently labelled samples for microarray hybridisation, we used a direct labelling protocol.
The central assumption of this study is that heterologous microarray hybridisation can be used to generate useful data with probes from closely related species.
Therefore, using microarray hybridisation analysis this complex pattern of gene expression can be monitored simultaneously.
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