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We melded in silico genome-wide PAP-derived predictions together with an empirically-determined list of differentially-expressed gene sets identified by microarray expression analysis to identify transcription factors (TFs) that regulate other transcription factors.
By microarray expression analysis, we have determined a gene signature for these Lgr5 stem cells.
The transcript is enriched in the endplate band of muscle compared with extrasynaptic regions, as shown by microarray expression analysis of microdissected samples (Kishi et al., 2005).
We therefore investigated the expression of IGF family members, their receptors and IGFBPs by microarray expression analysis of sural nerve biopsies from patients with DNP and age-matched control individuals (electronic supplementary Table A1).
To identify an EV biomarker for glioblastoma multiforme, serum MVs were analyzed by microarray expression analysis, and a group of RNAs that are upregulated or downregulated in this tumor type was identified [ 29, 55].
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We then extended our study by applying microarray expression analysis to a cohort of 98 CRC patients (Table 1).
ASCL1 target genes in the pancreatic endocrine tumour cell line BON1 were identified by RNA interference and microarray expression analysis.
Single-nucleotide polymorphisms can also be chosen from a region previously identified by linkage study or microarray expression analysis.
These cell proliferation markers (amongst others) are also upregulated in Wnt10b-driven tumours, as shown by microarray gene expression analysis and verifying qt-PCR (Supporting Information Fig S3A and B).
For example Bild et al. developed cell models representing candidate metastatic pathways, defined characteristic gene sets by microarray gene expression analysis, and demonstrated prognostic value in the prognostic data sets performed with gene expression [ 14].
Real time PCR, immunofluorescence and immunoblot analyses, performed by using probes against eight arbitrarily selected gene products out of thirty-four, confirmed the differential regulation determined by microarray gene expression analysis.
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