Sentence examples for by microarray experiments were from inspiring English sources
Results obtained by microarray experiments were validated by quantitative RT-PCR for some selected deregulated genes (CDH11, TFF3, C FAM5C, IL6R, KCMNA1) (data not shown).
The results obtained by microarray experiments were verified by real-time quantitative PCR (qPCR) by using the LightCycler 2.0 instrument and LightCycler® FastStart DNA MasterPLUS SYBR Green I (Roche Diagnostics GmbH, Manheim, Germany) as previously described [ 77].
The trends in the expression of these miRNAs detected by microarray experiments were consistent (7 miRNAs) or partially (5 miRNAs) consistent with those determined in stem-loop qRT-PCR analyses.
Even though the fold changes seen by microarray experiments is typically modest, such experiments may underestimate actual changes in malignant epithelial cells due to the dilution of changes in malignant epithelial cells by stromal cells that constitute a significant proportion of pathological specimens.
The difficulty of interpreting the lists of significant genes produced by microarray experiments is a major challenge.
Analysis of data obtained by microarray experiments was carried out by means of hierarchical gene clustering [ 18] using Cluster 3.0 (open source 2006) and TreeView (Stanford University Labs) software.
High-throughput transcriptomic data generated by microarray experiments is the most abundant and frequently stored data type currently used in translational studies.
High-throughput transcriptomic data generated by microarray experiments is the most abundant and frequently stored kind of data currently used in translational medicine studies.
In all cases, the quantitative RT-PCR validation indicated that differential expression detected by microarray experiment was highly reliable.
Changes in expression levels of 80 selected L. infantum and/or L. major mRNAs in either promastigotes or intracellular amastigotes as determined by the microarray experiments were confirmed by qRT-PCR.
The sequences of differentially expressed genes identified by the microarray experiments were collected from NCBI GeneBank [ 39] and compared them to known sequences from Cotton Gene Index [ 40] using the Basic Local Alignment Search Tool (BLAST) to determine if there was any significant homology to known gene products.
