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Frequency distribution plots of sequencing read counts for the Even, 4x, 16x, 64x and 2-20x poolshowow similar characteristics to the distributions generated for the same pools by microarray detection on the GMAP with some exceptions.
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The selected tags in single sperm from six unrelated healthy donors were amplified by multiplex PCR followed by microarray detection.
This was followed by a one hour microarray detection of the 48 samples on one standard slide.
The concentration of secretomes may be instantly measured by the microfluidic SPR microarray detection unit that is linked to the gut-on-a-chip (Fig. 4b) [86].
Both protocols are based on padlock probe ligation combined with microarray detection.
RNA was isolated from each half-ovary sent to CapitalBio for microarray detection and validation of the microarray data by quantitative real-time polymerase chain reaction (qRT-PCR).
The underlying principle of a microarray detection strategy is to estimate the number or amount of target by the interaction between immobilized probe on solid surface (e.g., glass slide or nylon membrane) and fluorescently labeled target.
Others combined PCR analysis with microarray detection.
Further, gene expression was analyzed by microarray studies on MCF-7 cell lines.
We developed a high throughput quantitative microarray antibody capture (Q-MAC) assay for anti-HDV IgG wherein recombinant HDV delta antigen is printed by microarray on slides coated with a noncontinuous, nanostructured plasmonic gold film, enabling quantitative fluorescent detection of anti-HDV antibody in small aliquots of patient serum.
Differences in protein detection by mass spectrometry and transcript detection by microarray were also explored.
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