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Heteroplasmy was screened by microarray calculation with the method of Coon et al. [65].
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The ND4L T10652C variant was confirmed by direct sequencing of mtDNA for the highest heteroplasmic individual (81% C heteroplasmy by microarray calculation) within the D1 haplogroup who was an MDD subject and had the C allele (Fig. 2).
There were 17 subjects within these haplogroups that had a heteroplasmy level greater than 50% by microarray calculation as previously described [65].
Here we describe the creation of miniaturised microarrays, 'mesoarrays', using DPN with protein spots 400× smaller by area compared to conventional microarrays.
Paternity was confirmed using identity by state calculation with PLINK.
We addressed this by microarrays with mRNA extracted from ammonium- or nitrate-adapted plants and validated these data by qPCR.
Several studies have evaluated gene expression by microarray for diagnostic purposes in adults and children with ARI and febrile illness.
We found that DR6 was highly expressed in human pDCs comparing with other blood cells by microarray analysis (Fig. 1A).
Comparison of 42 samples examined by both RNA-seq and microarray revealed marked differences in sensitivity, with transcripts identified only by RNA-seq having much lower expression than those also identified by microarray.
We transfected HEK293T cells with epitope-tagged Ago2, immunopurified Ago2 together with any associated miRNAs and mRNAs, and quantitatively determined the levels of these RNAs by microarray analyses.
Monocyte transcripts were analyzed by microarray and quantitative PCR.
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