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Recently, it has been shown by microarray assays that many of the Brachypodium CBF genes are cold-responsive [ 30].
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MiRNA and mRNA expressions were screened by microarray assays.
We identified 62 miRNAs as differentially expressed miRNAs by microarray assays.
Indeed, the quality of data generated by microarray assays has been questioned [ 1, 2].
Our results showed that gene expression patterns obtained by RT-qPCR analysis correlated to that observed by microarray assay.
If "positive genes" are sought by microarray assay, many such stimulated genes will be selected.
The genes investigated by quantitative PCR generally confirmed the changes obtained by microarray assay.
Numerous studies have demonstrated dysregulation of miRs in tumors by microarray assay [ 15, 16].
All four candidate genes demonstrated the same expression trends to those identified by microarray assay.
We compared the ratio of Cy5/Cy3 intensity of each clone obtained from the regular cDNA microarray assays to that obtained from the SSH/microarray assays.
Validation of clinical microarray-based assays that segregate patients with such narrow differences poses technical challenges.
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