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Gene expression profiles relative to vehicle-treated cells were assessed by microarray analysis with Affymetrix GeneChip 430 2.0 arrays.
The microarray results were validated by comparing the mean values for the expression data (log2 ratio) for genes from three independent replicates obtained by microarray analysis with the corresponding mean expression values obtained with the multiplex quantitative RT-PCR.
cChange in 16-h cultures of cepR, cciR or cepRcciIR mutants compared to 16-h cultures of K56-2 as determined by microarray analysis with at least two biological replicates (NC, no change).
aFunction derived from B. cenocepacia J2315 [ 34]. bChange in 16-h cultures of cepR, cciR or cepRcciIR mutants compared to 16-h cultures of K56-2 as determined by microarray analysis with at least two biological replicates (NC, no change).
Similarly, regulation of TXNIP and STS as identified by microarray analysis with a decrease after RASFsn stimulation and an increase after treatment with MTX, diclofenac, and prednisolone was also confirmed by PCR.
Notably, the mRNA encoding the proteasome subunit Rpn5p, which was found in both complexes by microarray analysis with similar enrichment factors (eIF3e = 5.1; eIF3m = 8.1) was also confirmed as being associated with both eIF3 complexes by RT-PCR (Fig. 5B).
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Proteins involved in nitrate/nitrite transport, such as NarX have been previously identified in a hypoxia model by microarray analysis (along with narK2), as well as by in situ hybridization in human lungs [50], [51].
Table S2 List of genes downregulated on Rifampicin treatment determined by Microarray analysis along with the number of DAF-16 binding sites in their 5 kb upstream region.
Table s1 List of genes upregulated on Rifampicin treatment determined by Microarray analysis along with the number of DAF-16 binding sites in their 5 kb upstream region.
As already determined by microarray analysis, treatment with the examined antirheumatic drugs exclusive of chloroquine phosphate and diclofenac significantly repressed the synthesis of CCL-20/MIP-3α in human chondrocytes.
Using qRT-PCR, we evaluated transcript levels of FZD6 (Frizzled 6), since levels measured by microarray analysis correlated with genital phenotype.
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