Fifty of 53 (94%) gender-regulated candidate genes identified by microarray analysis were confirmed by real-time RT-PCR.
Importantly, the altered gene patterns identified by microarray analysis were confirmed by RT-PCR and real-time RT-PCR.
CNVs identified by microarray analysis were validated by Multiplex Ligation-dependent Probe Amplification (MLPA).
To examine if decreases detected by microarray analysis were demonstrable by QPCR, one Group III case, DA38, was employed.
Several of the up and downregulated genes identified by microarray analysis were validated using qReal-time RT-PCR.
Selected dysregulated miRNA identified by microarray analysis were also confirmed to be dysregulated by qRT-PCR analysis.
Reductions in gene expression detected by microarray analysis were subtle, and differences never reached a two-fold decrease.
All genes that were downregulated in the Ts1Cje neurospheres by microarray analysis were also downregulated by RT-qPCR (Aurka, Brca2, Ccnb1, Ccna2, Cenpo, Mcm7, Pbk, Prim1 and Suv39h1).
Only three Hnf4α microarray bound regions and seven histone-marked regions identified by microarray analysis were not subsequently identified using the corresponding ChIP-seq data.
Gene expression results obtained by microarray analysis were confirmed for Cxcl10, Ccl12, Cxcl1 and Cxcl2 by Real-Time PCR (Figure 3).
However, levels of the TNF transcript as determined by microarray analysis were similar to those previously obtained by qPCR [12], validating monocyte activation by CEsHUT and inhibition by HDL in the 3 experiments.
