Sentence examples for by microarray analysis using from inspiring English sources

To clarify the onset of Fe deficiency, changes in gene expression were examined by microarray analysis using rice roots at 3, 6, 9, 12, 24, and 36 h after the onset of Fe-deficiency treatment.

Finally, the off-target effects of all siRNAs were determined by microarray analysis using Illumina Beadchip technology.

The transcriptional profiles were then determined by microarray analysis using Agilent human 44k 60-mer oligonucleotide microarray chips.

Following incubation with the extract, total RNA was purified from the cells and mRNA expression levels were evaluated by microarray analysis using an Affymetrix platform.

Transcriptional differences between the different groups and within individuals before and after the RR are assessed by microarray analysis using Affymetrix HG-U133 Plus 2.0 genechips (www.affymetrix.com).

Changes in global gene expression were quantified by microarray analysis using DU145 cells treated with TNFα, FGF19, or both, and harvested at 1.5 h.

Gene expression profiling by mRNA microarray analysis using whole testis RNA identified 619 genes differentially expressed (381 downregulated and 238 upregulated, P<0.001, FDR<0.04) in Ing2−/− testes compared with Ing2+/+ testes (GSE18610 at http://www.ncbi.nlm.nih.gov/geo/).nih.gov/geo/

The transcriptional profile of the excised tumors was characterized by oligonucleotide microarray analysis using the human U133A plus 2.0 Affymetrix GeneChip, according to previously described protocols for total RNA extraction and purification, cDNA synthesis, in vitro transcription for production of biotin-labeled cRNA, hybridization and scanning of image output files [26].

It was confirmed by DNA microarray analysis using custom-made inkjet-printed 16K oligonucleotide chips (Gene Expression Omnibus platform accession number GPL254: http://www.ncbi.nlm.nih.gov/projects/geo/) that the transfection of siRNA against Gαi2 or Gβ2 showed significant levels of the target gene knockdown (Figure 2).

Many previous studies have attempted to identify NATs by DNA microarray analysis using cDNA-oriented custom microarrays or commercially available microarray platforms [ 37- 41].

Each group had nine rats, and miRNA expression profiling was done by miRNA microarray analysis using a chip containing 300 mature miRNAs (Chip ID miRRat 9.2 version; LC Sciences).