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To investigate the cytokine response induced by immuno-stimulatory herbal extracts, PBMCs were treated with an herbal extract followed by microarray analysis on isolated cellular RNA.
The transcriptomes of mature, fully light-exposed leaves of soil grown P. euphratica and P. × canescens plants were compared by microarray analysis on GeneChip Poplar Genome arrays.
Quantitative RT-PCR on 8 EGFR mutated samples confirmed results obtained by microarray analysis on the five differentially expressed genes discussed above (figure 7).
Possible genetic compensation was assessed by microarray analysis on hippocampal cDNA of Kv10.1+/+ and Kv10.1−/− (Supplementary Material, Table S1); except for the targeted gene itself, no significant differences were observed between the samples.
The chromosomal distribution of these genes is similar to the distribution determined by microarray analysis on germline-enriched genes [ 11, 12]; germline-expressed genes are over-represented on chromosomes I and III, and under-represented on chromosomes V and X, assuming a random distribution of germline-expressed genes in the genome.
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Next we sought to expand on our findings obtained by microarray analysis of starved whole larvae by focusing on the fat body.
For example, chromatin immunoprecipitation studies followed by microarray analysis (ChIP-on-chip) of genes regulated by related estrogen receptors in estrogen-sensitive breast carcinoma cells have suggested that only a minority of potential high affinity estrogen response elements of a given chromosome are accessible to receptors [ 27].
They also demonstrated that 20% of the S. mutans UA159 open reading frames (ORFs) were absent from one or more of the nine test strains, and dispensable ORF blocks (including more than one ORF) were identified by microarray analysis based on the UA159 genome [ 28].
The proteomic observations were supported in part by microarray analysis performed on the mRNA isolated from chondrocytes extracted from the cartilage of 5-day-old mice.
These findings are supported by microarray analysis conducted on cardiomyocytes subjected to hESC-conditioned medium, demonstrating that MAPK pathway signaling was among the main induced signaling cascades [ 22].
In order to identify these genes, we compared the gene expression patterns, obtained by microarray analysis performed on the Affymetrix platform.
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