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(b) Relative gene expression as determined by microarray analysis for several candidate genes that map within the del 5q) region.
Using quantitative real-time RT-PCR we confirmed the expression patterns obtained by microarray analysis for selected genes related to regulation of gene transcription (TIGD4, HOXC10, CHMP4B and PCAF) and cytokine secretion (SOCS7).
miRNA profiles in the intracellular and extracellular fractions were determined by microarray analysis for seven cancer cell lines including AZ-521, AZ-P7a, SBC-3, NCI-H69, DMS53, SW480, and SW620.
We have tested candidate genes identified by microarray analysis for their ability to alter the patterning of the Drosophila eye.
bTranscript levels were measured by microarray analysis for subset 1 or qRT-PCR for subset 2. cMean ± standard deviation.
Transcriptional alterations related to HSF1 protein level were investigated by microarray analysis for 224 freshly frozen samples in parallel.
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The expression differences were of the same range values as those measured by microarrays analysis for WARS and SIAH2.
These are similar to a previous observation that genes identified in genetic screens are enriched for regulatory pathways, whereas differentially expressed genes identified by microarray analysis are enriched for metabolic pathways [ 15].
RBM24, which has been shown to be up-regulated by HCV infection by microarray analysis, was selected for further study.
Validation of the data obtained by microarray analysis is indispensable for identifying genes differentially expressed in cloned embryos.
Gene expression results obtained by microarray analysis were confirmed for Cxcl10, Ccl12, Cxcl1 and Cxcl2 by Real-Time PCR (Figure 3).
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