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The observation that glial markers were absent by microarray analysis (data not shown) indicates that few glial cells or oligodendrocytes were present in the neuronal cultures.
The pattern of expression of NF-κB signaling genes in RAW 264.7 cells after 60 min, 90 min and 180 min of LeTx exposure was determined by microarray analysis (data not shown).
The differentiated neurons expressed subunits for voltage-gated Na+, K+ and Ca2+ channels by microarray analysis (data not shown), indicating that the cells contain ion channels characteristic of neurons.
The RT PCR results of all 10 genes showed similar differential expression patterns to those obtained by microarray analysis (Data not shown).
RNA gel blot analysis of ten known genes and three unknowns (see Table 2) correlated with the general expression profile obtained by microarray analysis data.
Second, we repeated the mRNA tagging experiment and performed qPCR to analyze gene expression levels for more than 100 of the putative target genes identified by microarray analysis (data not shown).
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These RNA blots produced similar expression profiles to the ones observed by oligo microarray analysis (data not shown).
Moreover, no increased expression of ABCG2 (breast cancer resistance protein, BCRP) was observed in the NBs by DNA microarray analysis (data not shown).
This is further supported by the microarray analysis data with NB patient tumor samples, showing that high expression of USP7 predicts poor outcomes.
The activity of these promoter regions is in apparent discrepancy with the detection of AtMYB60 gene expression in seeds, as revealed by available microarray analysis data [ 33, 34] and in roots, as recently reported [ 35].
Nevertheless, in the present study no soluble factor was suggested to promote NF-25 fibroblast growth when separately co-cultured with HSC-39 cells, and increased mRNA levels of SDF-1 expression were not detected in NF-25 fibroblasts co-cultured with HSC-39 cells by cDNA microarray analysis (data not shown).
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