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SOX2 mRNA (probe sets 213721_at and 228038_at) was also compared by microarray analysis between formalin-fixed paraffin embedded (FFPE) lung SCCs and adenocarcinomas (unpublished observations).
The most prominent change in miRNA expression by microarray analysis between the three cell lines not able to colonize distant sites (67NR, 168FARN and 4TO7 cells) and 4T1 cells was up-regulation of several members of the miR-200 family in 4T1 cells (Figure 1A, Table S1).
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The sucrose induced changes in polysomal occupancy observed by microarray analysis range between 1.3 and 3.4 fold.
M. tuberculosis total RNA was successfully amplified and analysed by microarray analysis from between 5 to 500 ng of total RNA.
We were not able to demonstrate by microarray analysis a clear correlation between the expression of a particular miRNA and a predominating cell type.
Surprisingly, attempts to define matrix-dependent gene expression profiles by microarray analysis revealed minimal differences between Matrigel- and collagen I-cultured tumor fragments, suggesting that key mRNA differences may be restricted to a minority of cells or that the important alterations are post-translational.
For protein-coding genes, gene expression profiles were compared by DNA microarray analysis between the N-His-AdpA-producing Δ adpA strain and the Δ adpA strain containing the empty integration vector pTYM19 on the chromosome.
We exploited this database to define the presence of functional associations within the genes detected by microarray analysis and to identify differences between the ontological gene classes that were enriched among differ expressed genes.
To provide further support that the transcriptional profiles detected by microarray analysis accurately represented the differences between villus and crypt epithelial cells, we evaluated expression of selected genes by immunohistochemistry.
A comparison of the NCA-simulated gene expression levels with the original measurements as obtained by Yadav et al. [ 38] by microarray analysis, shows a good agreement between the two sets.
We exploited this database to define the presence of functional associations within the genes detected by microarray analysis and to identify differences between the ontological gene classes that were enriched among differentially expressed genes.
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