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On the other hand, genes identified by microarray analysis are expected to be a mix of causal genes and the genes that are differentially expressed because of the ripple effect of the causal genes.
Ten proteins involved in protein folding and chaperoning were found to be altered by the low protein diet in proteomic analysis [18] and data provided by microarray analysis are congruent with an increased sensitivity to oxidative stress in islets from LP fetuses [19].
The signal intensities of these select housekeeping genes, as determined by microarray analysis, are shown in Figure 1, Panel A. Signal intensities of these housekeeping genes were comparable for samples of EHEC O157∶H7 under each of the four different growth conditions.
Therefore, it is unlikely that the chloroplast genes identified by microarray analysis are functional targets of ABF3.
Indeed, the largest numbers of DEGs identified by microarray analysis are detected at this time point (Table 1).
If the genes identified by microarray analysis are actual downstream targets of ABF3, they would be expected to contain at least one ABRE.
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Fifty of 53 (94%) gender-regulated candidate genes identified by microarray analysis were confirmed by real-time RT-PCR.
Importantly, the altered gene patterns identified by microarray analysis were confirmed by RT-PCR and real-time RT-PCR.
RBM24, which has been shown to be up-regulated by HCV infection by microarray analysis, was selected for further study.
CNVs identified by microarray analysis were validated by Multiplex Ligation-dependent Probe Amplification (MLPA).
To examine if decreases detected by microarray analysis were demonstrable by QPCR, one Group III case, DA38, was employed.
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