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Expression ratios for individual genes were examined by microarray analyses (see Supporting Information, Table S1).
The relative expression level of each gene was highly correlated with the data obtained by microarray analyses (see Supplementary Fig. 2).
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All of the tested genes were selected based on the microarray analyses (see below).
Stella and Piwil2 were not increased in rES cells by microarray analyses (Figure S8B).
Expression of all genes found by microarray analyses to be differentially expressed 2-fold or greater was also analyzed by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR; see Table S2) to verify differential expression.
We compared OsDREB1A, AP59, OsbZIP23, SNAC1, OsNAC5 and OsNAC6 downstream genes identified by microarray analyses.
The RNAs were isolated and reverse transcribed into cDNAs, followed by microarray analyses.
Global gene expressions were measured by microarray analyses.
Furthermore, several HMGA1a-regulating genes and pathways have been suggested by microarray analyses [13].
As shown by microarray analyses the transcripts of their genes were up-regulated upon 'BN' phytoplasma infection [see Additional file 3].
We therefore validated our results by Microarray analyses.
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